Caracterização morfológica, fisiológica e infectividade em planta de estirpes de Frankia isoladas de nódulos de Casuarina / Morphological, physiological and plant infectivity characterization of Frankia strains isolated from Casuarina’s nodules

RESUMO

Frankia são microrganismos capazes de fixar N2 quando em simbiose com espécies arbóreas, denominadas plantas actinorrízicas. Este estudo foi conduzido para caracterizar morfologicamente, fisiologicamente e avaliar a nodulação de quatro estirpes referência de Frankia (HFPCcI3, JCT287, KB5 e F59) e 12 estirpes(IPRF) isoladas de nódulos de Casuarina. Todas as estirpes (Referência e IPRF) foram Gram-positivas e 50% foram “acid-fast”. As estirpes de Frankia alcalinizaram o meio de cultura, exceto IPRF006, IPRF008e IPRF010. As colônias das estirpes F59, IPRF002, IPRF004, IPRF005 e IPRF011, produziram melanina. Entre as estirpes referência, apenas a JCT287 cresceu no meio de cultura com pH 5,5, enquanto no meio com pH 6,0 as duas, JCT287 e KB5, apresentaram crescimento. A análise de regressão mostrou uma relação linear (Y = 67,56+ 3,88X e R2=0,5862, p<0,05) e coeficiente de correlação de Pearson (r=0,766,p<0,05) entre número de esporos e hifas (120 a 480 UFC mL-1) e proteína total (18 a 145 µg mL-1). Foi observado que a estirpe F59 apresentou uma maior proporção de proteína total (50%) do que a JCT287(7,0%) e, que as estirpes IPRF mostraram valores entre 17,5 e 29,3%. Todas as estirpes produziram compostos indólicos com valores variando de 5,9 a 98,8 µM.

ABSTRACT

Frankia are soil microorganisms that form symbiosis with roots of tree species called actinorhizal plants and are capable of fixing atmospheric N2. This study was carried out to characterize morphologically, physiologically and to assess the nodulation of four Frankia reference strains (HFPCcI3, JCT287, KB5and F59) and 12 (IPRF) isolated from root nodules of Casuarina plants. All strains (Reference and IPRF)were characterized as Gram-positive and 50% as acid-fast. The Frankia strains produced alkali in the culture medium, except the IPRF006, IPRF008 and IPRF010. The colonies of strains F59, IPRF002, IPRF004,IPRF005, and IPRF011 produced melanin. Among reference strains, only JCT287 grew in culture mediawith pH 5.5, while with pH 6.0 both strains JCT287 and KB5 presented growth. The regression analysis showed a linear relationship (Y = 67.56+ 3.88X and R2=0.5862, p<0.05) and Pearson’s correlation coefficient(r=0.766, p<0.05) between number of spores and hyphae (120 to 480 UFC mL-1) and total protein (18 to 145µg mL-1). It was observed that the strains F59 had a higher proportion of total protein 50.0% than JCT287with 7.0% and that the IPRF strains showed values between 17.5 and 29.3%. All strains presented ability to produce indolic compounds in growth media with values ranging from 5.9 to 98.8 µM.