á-N-acetylglucosamine-linked O-glycans of sialoglycoproteins (Tc-mucins) from Trypanosoma cruzi Colombiana strain
Mem. Inst. Oswaldo Cruz
;
104(supl.1): 270-274, July 2009. ilus, graf
Article
in English
| LILACS
| ID: lil-520901
ABSTRACT
Trypanosoma cruzi sialoglycoproteins (Tc-mucins) are mucin-like molecules linked to a parasite membrane via a glycosylphosphatidylinositol anchor. We previously determined the structures of Tc-mucin O-glycan domains from several T. cruzi strains and observed significant differences among them. We now report the amino acid content and structure of Tc-mucin O-glycan chains from T. cruzi Colombiana, a strain resistant to common trypanocidal drugs. Amino acid analysis demonstrated the predominance of threonine residues (42%) and helped to identify the O-glycans as belonging to a Tc-mucin family that contain a ²-galactofuranose (²-Galf) residue attached to an á-N-acetylglucosamine (á-GlcNAc) O-4, with the most complex glycan, a pentasaccharide-GlcNAc-ol with a branched trigalactopyranose chain, on the GlcNAc O-6. The presence of ²-Galf on O-glycans from T. cruzi Colombiana mucins supports the use of glycosylation as a phylogenetic marker for the classification of Colombiana in the T. cruzi I group.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Oligosaccharides
/
Acetylglucosamine
/
Sialoglycoproteins
/
Trypanosoma cruzi
/
Carbohydrate Conformation
/
Mucins
Country/Region as subject:
South America
/
Colombia
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Type:
Article
Affiliation country:
Brazil
/
United kingdom
Institution/Affiliation country:
National Institute for Biological Standards and Control/GB
/
Universidade Federal do Rio de Janeiro/BR
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