Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L)
Electron. j. biotechnol
;
11(1): 134-139, Jan. 2008. ilus, tab
Article
in English
| LILACS
| ID: lil-522167
ABSTRACT
Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the beta-glucuronidase (uid A) gene. Histochemical beta-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation.
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Index:
LILACS (Americas)
Main subject:
Transformation, Genetic
/
Agrobacterium tumefaciens
/
Argemone
Country/Region as subject:
Mexico
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2008
Type:
Article
/
Project document
Affiliation country:
Mexico
Institution/Affiliation country:
Centro de Investigación Científica de Yucatán/MX
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