Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
Electron. j. biotechnol
; 11(2): 56-68, Apr. 2008. ilus, graf, tab
Article
in En
| LILACS
| ID: lil-522211
Responsible library:
CL1.1
ABSTRACT
Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3 percent of the contigs and 88.5 percent of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature.
Full text:
1
Index:
LILACS
Main subject:
Gene Library
/
Cold Climate
/
Eucalyptus
Type of study:
Prognostic_studies
Language:
En
Journal:
Electron. j. biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2008
Type:
Article
/
Project document