Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
Electron. j. biotechnol
;
11(2): 56-68, Apr. 2008. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-522211
ABSTRACT
Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3 percent of the contigs and 88.5 percent of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Gene Library
/
Cold Climate
/
Eucalyptus
Type of study:
Prognostic study
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2008
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Universidad Andres Bello/CL
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