Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods
Mem. Inst. Oswaldo Cruz
;
104(4): 576-579, July 2009. ilus, tab
Article
in English
| LILACS
| ID: lil-523722
ABSTRACT
The aim of this study was to compare two nucleic acid extraction methods for the recovery of enteric viruses from activated sludge. Test samples were inoculated with human adenovirus (AdV), hepatitis A virus (HAV), poliovirus (PV) and rotavirus (RV) and were then processed by an adsorption-elution-precipitation method. Two extraction methods were used: an organic solvent-based method and a silica method. The organic-based method was able to recoup 20 percent of the AdV, 90 percent of the RV and 100 percent of both the PV and HAV from seeded samples. The silica method was able to recoup 1.8 percent of the AdV and 90 percent of the RV. These results indicate that the organic-based method is more suitable for detecting viruses in sewage sludge.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
RNA Viruses
/
Sewage
/
Water Microbiology
/
Adenoviruses, Human
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Santa Catarina/BR
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