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In vitro antimicrobial activity of acroseal, polifil and epiphany against Enterococcus faecalis
Pinheiro, Cláudia Ramos; Guinesi, Adriana Simionatto; Pizzolitto, Antônio Carlos; Bonetti-Filho, Idomeo.
  • Pinheiro, Cláudia Ramos; São Paulo State University. Araraquara Dental School. Department of Restorative Dentistry. Araraquara. BR
  • Guinesi, Adriana Simionatto; São Paulo State University. Araraquara Dental School. Department of Restorative Dentistry. Araraquara. BR
  • Pizzolitto, Antônio Carlos; São Paulo State University. School of Pharmaceutical Sciences. Departament of Clinical Analysis. Araraquara. BR
  • Bonetti-Filho, Idomeo; São Paulo State University. Araraquara Dental School. Department of Restorative Dentistry. Araraquara. BR
Braz. dent. j ; 20(2): 107-111, 2009.
Article in English | LILACS | ID: lil-524520
ABSTRACT
Using the agar diffusion method, this study evaluated the in vitro antimicrobial activity of the commercial endodontic sealers Acroseal and Epiphany, a castor-oil based experimental sealer, Polifil, and a primer agent (Epiphany self-etching primer), against Enterococcus faecalis. Zinc oxide and eugenol cement (ZOE) served as control. Five wells per dish were made at equidistant points and immediately filled with the test and control materials. After incubation of the dishes at 37ºC for 24 h and 48 h, the diameter of the zones of microbial growth inhibition produced around the wells was measured (in mm) with a millimeter rule. After 48 h, the diameters of the zones of microbial growth inhibition were the same as those observed at 24 h, only the substances continued to diffuse. Epiphany and Polifil did not show antibacterial activity (no formation of zones of microbial growth inhibition). The primer produced the largest zones of inhibition (17.62 mm) followed by Acroseal (7.25 mm) and ZOE (7.12 mm). E. faecalis was resistant to Epiphany and Polifil, while the primer and Acroseal sealer were effective against this microorganism under the tested conditions.
RESUMO
Utilizando o método de difusão em ágar, este estudo avaliou in vitro a atividade antimicrobiana dos cimentos endodônticos comerciais Acroseal e Epiphany, um cimento experimental à base do polímero da mamona, Polifil, e um agente primer (primer auto-adesivo do sistema Epiphany), contra Enterococcus faecalis. O cimento de óxido de zinco e eugenol (OZE) serviu como controle. Cinco poços por placa foram feitos em pontos eqüidistantes e imediatamente preenchidos com os materiais testados e controle. Após a incubação das placas aos 37ºC por 24 e 48 h foram medidas as zonas de inibição do crescimento bacteriano ao redor dos poços (em mm) com uma régua milimetrada. Após 48 h, os diâmetros das zonas de inibição do crescimento bacteriano foram os mesmos observados às 24 h, apenas as substâncias continuaram a se difundir. Epiphany e Polifil não apresentaram atividade antibacteriana (não ocorreu a formação das zonas de inibição do crescimento bacteriano). Os maiores halos de inibição foram atribuídos ao primer (17,62 mm), seguido do Acroseal (7,25 mm) e OZE (7,12 mm). E. faecalis foi resistente ao Epiphany e Polifil, enquanto que o primer e o cimento Acroseal foram eficientes contra esse microrganismo nas condições testadas.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Root Canal Filling Materials / Enterococcus faecalis Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2009 Type: Article Affiliation country: Brazil Institution/Affiliation country: São Paulo State University/BR

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Full text: Available Index: LILACS (Americas) Main subject: Root Canal Filling Materials / Enterococcus faecalis Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2009 Type: Article Affiliation country: Brazil Institution/Affiliation country: São Paulo State University/BR