Infection kinetics of human adenovirus serotype 41 in HEK 293 cells
Mem. Inst. Oswaldo Cruz
;
104(5): 736-744, Aug. 2009. ilus
Article
in English
| LILACS
| ID: lil-528083
ABSTRACT
The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Adenoviruses, Human
Limits:
Animals
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Type:
Article
/
Project document
Affiliation country:
Brazil
/
France
Institution/Affiliation country:
Institut de Biologie Structurale Jean-Pierre Ebel/FR
/
Universidade de São Paulo/BR
/
Universitaire de Genoble/FR
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