New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
Braz. j. microbiol
;
40(4): 778-781, Oct.-Dec. 2009. ilus
Article
in English
| LILACS
| ID: lil-528159
ABSTRACT
We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
In Vitro Techniques
/
Base Sequence
/
Proteins
/
Gene Expression
/
Cloning, Molecular
/
Escherichia coli
/
DNA Fragmentation
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Goiás/BR
/
Universidade Federal do Amazonas/BR
/
Universidade de Brasília/BR
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