Isolation and identification of profenofos degrading bacteria
Braz. j. microbiol
;
40(4): 893-900, Oct.-Dec. 2009. graf, tab
Article
in English
| LILACS
| ID: lil-528172
ABSTRACT
An enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from Hubei province of central China. Two pure bacterial cultures, named W and Y, were isolated and subsequently characterized by sequencing of 16S rRNA genes and biochemical tests. Isolate W showed 96 percent similarity to the 16S rRNA gene of a Pseudomonas putida unlike Y which showed 99 percent similarity to the 16S rRNA gene of Burkholderia gladioli. Both strains grew well at pH 5.5-7.2 with a broad temperature profile ranging from 28º to 36 ºC. Bioremediation of profenofos-contaminated soil was examined using soil treated with 200 ug g-1; profenofos resulted in a higher degradation rate than control soils without inoculation. In a mineral salt medium (FTW) reduction in profenofos concentration was 90 percent within 96 hours of incubation. A literature survey revealed that no data is available regarding the role of Burkholderia gladioli on pesticide biodegradation as well as on profenofos.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
In Vitro Techniques
/
RNA, Bacterial
/
Base Sequence
/
Burkholderia Infections
/
Burkholderia gladioli
/
Insecticides, Organophosphate
Type of study:
Diagnostic study
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2009
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
China University of Geosciences/CN
Similar
MEDLINE
...
LILACS
LIS