Glass bead transformation method for gram-positive bacteria

Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat

Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat.

Rattanachaikunsopon, Pongsak; Ubon Ratchathani University. Department of Biological Science. Ubon Ratchathani. TH
Phumkhachorn, Parichat; Ubon Ratchathani University. Department of Biological Science. Ubon Ratchathani. TH

Braz. j. microbiol ; 40(4): 923-926, Oct.-Dec. 2009. graf, tab

Article in English | LILACS | ID: lil-528176

ABSTRACT

ABSTRACT

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.

Subject(s)

Gram-Positive Bacteria/genetics; Genetics, Microbial; Protoplasts; Transformation, Bacterial; Glass/analysis; Methods; Methods

Glass bead; Gram-positive bacteria; Transformation

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Full text: Available Index: LILACS (Americas) Main subject: Protoplasts / Transformation, Bacterial / Genetics, Microbial / Glass / Gram-Positive Bacteria Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2009 Type: Article Affiliation country: Thailand Institution/Affiliation country: Ubon Ratchathani University/TH

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