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Glass bead transformation method for gram-positive bacteria
Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat.
Affiliation
  • Rattanachaikunsopon, Pongsak; Ubon Ratchathani University. Department of Biological Science. Ubon Ratchathani. TH
  • Phumkhachorn, Parichat; Ubon Ratchathani University. Department of Biological Science. Ubon Ratchathani. TH
Braz. j. microbiol ; Braz. j. microbiol;40(4): 923-926, Oct.-Dec. 2009. graf, tab
Article in En | LILACS | ID: lil-528176
Responsible library: BR32.1
ABSTRACT
A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.
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Full text: 1 Index: LILACS Main subject: Protoplasts / Transformation, Bacterial / Genetics, Microbial / Glass / Gram-Positive Bacteria Language: En Journal: Braz J Microbiol / Braz. j. microbiol / Braz. j. microbiol. (Online) / Brazilian journal of microbiology (Impresso) Journal subject: MICROBIOLOGIA Year: 2009 Type: Article
Fulltext
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Full text: 1 Index: LILACS Main subject: Protoplasts / Transformation, Bacterial / Genetics, Microbial / Glass / Gram-Positive Bacteria Language: En Journal: Braz J Microbiol / Braz. j. microbiol / Braz. j. microbiol. (Online) / Brazilian journal of microbiology (Impresso) Journal subject: MICROBIOLOGIA Year: 2009 Type: Article