The contribution of genes required for anaerobic respiration to the virulence of Salmonella enterica serovar Gallinarum for chickens
Braz. j. microbiol
;
40(4): 994-1001, Oct.-Dec. 2009. tab
Article
in English
| LILACS
| ID: lil-528185
ABSTRACT
Salmonella enterica serovar Gallinarum (SG) is an intracellular pathogen of chickens. To survive, to invade and to multiply in the intestinal tract and intracellularly it depends on its ability to produce energy in anaerobic conditions. The fumarate reductase (frdABCD), dimethyl sulfoxide (DMSO)-trimethylamine N-oxide (TMAO) reductase (dmsABC), and nitrate reductase (narGHIJ) operons in Salmonella Typhimurium (STM) encode enzymes involved in anaerobic respiration to the electron acceptors fumarate, DMSO, TMAO, and nitrate, respectively. They are regulated in response to nitrate and oxygen availability and changes in cell growth rate. In this study mortality rates of chickens challenged with mutants of Salmonella Gallinarum, which were defective in utilising anaerobic electron acceptors, were assessed in comparison to group of bird challenged with wild strain. The greatest degree of attenuation was observed with mutations affecting nitrate reductase (napA, narG) with additional attenuations induced by a mutation affecting fumarate reductase (frdA) and a double mutant (dmsA torC) affecting DMSO and TMAO reductase.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Poultry
/
Salmonella Infections
/
Bacteria, Anaerobic
/
Salmonella enterica
/
Enzyme Activation
/
Genes, Bacterial
/
Mutation
Type of study:
Evaluation studies
Limits:
Animals
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2009
Type:
Article
Affiliation country:
Brazil
/
United kingdom
Institution/Affiliation country:
Universidade Estadual Paulista/BR
/
University of Nottingham/GB
Similar
MEDLINE
...
LILACS
LIS