Characterization of fractions of aqueoussoluble constituents of the mushroom Pleurotus ostreato-roseus Sing. and its effects on the serum protein profile of mice

ABSTRACT

Various species of mushrooms belonging to genus Pleurotus (Fr.) Kumm. are known by its nutritional value and medicinal properties, particularly anti-tumor activity and immunomodulation, related to structural polysaccharides. In order to study the composition and the effects of aqueoussoluble constituents from Pleurotus ostreato-roseus Sing. on mice serum proteins, the P. ostreato-roseus strain POR-020 was cultured on powered grass-sugarcane bagasse-sawdust-wheat bran-substrate, and fruiting bodies were produced, milled, extracted with ethanol and hot water, and fractional precipitated with ethanol producing two fractions, FI-1 and FI-2. These fractions showed, respectively, 47.87 and 26.45 percent (d. m.) carbohydrate, and 2.07 and 1.97 percent (d.m.) protein composition. The fractions were administered i.p. in AJ male mice in a singledose of 257 mg/kg (FI-1) and 31.5 mg/kg (FI-2). After 15 days administration, electrophoretic analysis of serum proteins detected that FI-1 increased 50, 25, 0.35, and 28.6 percent in b-, a1-, a2- and g-globulin regions, and FI-2 increased 4.4 and 21 percent in band a1-globulin, respectively.