Characterization of indigenous rhizobia from Caatinga
Braz. j. microbiol
;
41(1): 201-208, Jan.-Mar. 2010. ilus, graf
Article
in English
| LILACS
| ID: lil-531753
ABSTRACT
The aim of this study was to characterize rhizobial isolates from Cratylia mollis Mart. ex Benth, Calliandra depauperata Benth. and Mimosa tenuiflora (Willd.) Poir. by means of rhizobial colonies morphology and restriction analysis of the 16S ribosomal gene (16S rDNA-ARDRA). Nodules were collected in the field and from plants cultivated in a greenhouse experiment using Caatinga soil samples. Sixty seven isolates were described by morphological analysis. Forty seven representative isolates were used for ARDRA analysis using seven restriction enzymes. We observed high diversity of both slow and fast-growing rhizobia that formed three morpho-physiological clusters. A few fast-growing isolates formed a group of strains of the Bradyrhizobium type; however, most of them diverged from the B. japonicum and B. elkanii species. Cratylia mollis nodule isolates were the most diverse, while all Mimosa tenuiflora isolates displayed fast growth with no pH change and were clustered into groups bearing 100 percent similarity, according to ARDRA results.
Full text:
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Index:
LILACS (Americas)
Main subject:
Rhizobiaceae
/
In Vitro Techniques
/
Enzyme Activation
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2010
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro Nacional de Pesquisa de Agrobiologia/BR
/
Fundação Instituto Brasileiro de Geografia e Estatística/BR
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