Isolation and fusion of protoplasts from the phytopathogenic fungus Sclerotium rolfsii (Sacc. )
Braz. j. microbiol
;
41(1): 253-263, Jan.-Mar. 2010. ilus, graf
Article
in English
| LILACS
| ID: lil-531759
ABSTRACT
Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5) /g mycelia and 2.8x10(5) /g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave maximum yield. Lysing enzyme at the rate of 15mg/ml was found best for yield. Fusion of protoplasts from strains A and R was carried out in fusion media containing PEG 4000 30 percent (w/v) with 0.2mM CaCl2. Four fusants F1, F2, F3 and F4 were recovered. Morphological, physiological and pathogenic characters of fusants were compared with parent strains on carrots, beans and tomato.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Protoplasts
/
Ascomycota
/
Basidiomycota
/
Enzyme Stability
/
Gene Fusion
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2010
Type:
Article
Affiliation country:
Canada
/
Greece
Institution/Affiliation country:
Fellow Alberta Research Council/CA
/
University of Patras/GR
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