Biotransformation of 3-hydroxydibenzo-alpha-pyrone into 3, 8 dihydroxydibenzo-alpha-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native shilajit
Electron. j. biotechnol
;
11(3): 1-10, July 2008. ilus, graf
Article
in English
| LILACS
| ID: lil-531898
ABSTRACT
Shilajit is a panacea in Ayurveda, the Indian traditional system of medicine. The major bioactives of shilajit have been identified as dibenzo-alpha-pyrones (DBPs), its oligomers and aminoacyl conjugated derivatives. These bioactive compounds play a crucial role in energy metabolism in all animal cells including those of man. 3-hydroxydibenzo-alpha-pyrone (3-OH-DBP), a key DBP component of shilajit is converted, among other products, to another active DBP derivative, viz. 3,8-hydroxydibenzo-alpha-pyrone, 3,8(OH)2-DBP, in vivo, when its precursor is ingested. 3,8(OH)2-DBP is then involved in energy synthesis in the mitochondria in the reduction and stabilization of coenzyme Q10 in the electron transport chain. As the chemical synthesis of 3,8(OH)2-DBP is a complex, multi-step process and economically not readily viable, we envisioned the development of a process using microorganisms for bioconversion of 3-OH-DBP to 3,8(OH)2-DBP. In this study, the biotransformation of 3-OH-DBP is achieved using Aspergillus niger, which was involved in the humification process on sedimentary rocks leading to shilajit formation. A 60 percent bioconversion of 3-OH-DBP to 3,8(OH)2-DBP and to its aminoacyl derivatives was achieved. The products were characterized and estimated by high performance liquid chromatography (HPLC), high performance flash chromatography (HPFC) and gas chromatography-mass spectrometry (GC-MS) analyses. Among the Aspergillus species isolated and identified from native shilajit, A. niger was found to be the most efficient for this bioconversion.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Organic Chemicals
/
Plants, Medicinal
/
Aspergillus niger
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2008
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
Jadvapur University/IN
/
Natreon Inc/IN
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