Cloning of a novel acidic phospholipase A2 from the venom gland of Crotalus durissus cascavella (Brazilian northeastern rattlesnake)
J. venom. anim. toxins incl. trop. dis
;
15(4): 745-761, 2009. ilus
Article
in English
| LILACS
| ID: lil-532757
ABSTRACT
The phospholipase A2 superfamily encompasses 15 groups that are classified into secreted PLA2 (sPLA2); cytosolic PLA2 (cPLA2); Ca2+-independent intracellular PLA2 (iPLA2); platelet-activating factor acetylhydrolase (PAF-AH); and lysosomal PLA2. Currently, approximately 700 PLA2 sequences are known, of which 200 are obtained from the venom gland of Crotalinae snakes. However, thus far, little information is available on cloning, purification and structural characterization of PLA2 from Crotalus durisssus cascavela venom gland. In the present work, we report the molecular cloning of a novel svPLA2 from C. d. cascavella (Cdc), a predominant rattlesnake subspecies in northeastern Brazil. The Cdc svPLA2 cDNA precursor is 689 nucleotides long and encodes a protein of 138 amino acid residues, with a calculated molecular mass of approximately 13,847 Da and an estimated isoelectric point of 5.14. Phylogenetic analysis of Crotalinae PLA2 reveals that Cdc PLA2 clustered with other acidic type IIA PLA2 homologues is also present in the venom of North American rattlesnakes. Hitherto, this study presents a novel PLA2 cDNA precursor from C. d. cascavella and data reported herein will be useful for further steps in svPLA2 purification and analysis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cloning, Molecular
/
Crotalid Venoms
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Butantan Institute/BR
/
Federal University of Pernambuco/BR
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