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Experimental splenectomies and malaria in mice / Esplenectomias e malária experimental em camundongos
Negreiros, Róbson Miguel de Araújo; Makimoto, Fabiano Hiromichi; Santana, Linda Luciana Oliveira; Ferreira, Luís Carlos de Lima; Nakajima, Gerson Suguiyama; Santos, Maria Cristina dos.
  • Negreiros, Róbson Miguel de Araújo; UFAM. School of Medicine. Surgery Clinical Department. Manaus. BR
  • Makimoto, Fabiano Hiromichi; UFAM. School of Medicine. Manaus. BR
  • Santana, Linda Luciana Oliveira; UFAM. School of Medicine. Manaus. BR
  • Ferreira, Luís Carlos de Lima; UFAM. School of Medicine. Pathology Department. Manaus. BR
  • Nakajima, Gerson Suguiyama; UFAM. School of Medicine. Surgery Clinical Department. Manaus. BR
  • Santos, Maria Cristina dos; UFAM. Biological Sciences Institute. Parasitology Department. Manaus. BR
Acta cir. bras ; 24(6): 437-441, Nov.-Dec. 2009. graf
Article in English | LILACS | ID: lil-533204
ABSTRACT

PURPOSE:

To evaluate the importance of spleen in malaric infection in murino model, comparing the parasitemia and the titles of imunoglobulins in the different groups.

METHODS:

It was used female mice non-isogenic, in inoculated with Plasmodium berghei, cepa ANKA, intraperitoneally. The parasitemia was analyzed in 23rd, 25th, 27th and 32nd day of the experiment, being the stained blood' exam colored by Giemsa. The titles of the total serum immunoglobulins IgM and IgG were analyzed by Dot-ELISA technique, at 6th, 22nd and 32nd day, when the animals were sacrificed.

RESULTS:

The parasitemia was gradual in all the inoculated groups. In the end of the experiment, the animals with partial parasitemia present superior parasitemia, but next to the non-splenectomized, while the asplenics present difference bigger than the double. The levels of total serum IgM and IgG didin´t have significant changes with the removal partial or total splenic.

CONCLUSION:

The techniques conservatives in splenic trauma are possible and necessary. The importance of remaining spleen in the clearance of red blood cells parasitized by Plasmodium berghei showed being efficient, in order to avoid serious complications resulting of the malaria in mice.
RESUMO

OBJETIVO:

Avaliar a importância do baço na infecção malárica em modelo murino, comparando a parasitemia e os títulos das imunoglobulinas nos diferentes grupos.

MÉTODOS:

Utilizaram-se camundongos fêmeos não isogênicos, inoculados com Plasmodium berghei, cepa ANKA, intraperitoneal. A parasitemia foi analisada no 23°, 25°, 27º e 32° dia do experimento, sendo o exame do esfregaço sangüíneo, corado pelo Giemsa. As titulações das imunoglobulinas totais séricas IgM e IgG foram realizadas pela técnica Dot-ELISA, no 6°, 22° e no 32° dia, quando os animais foram sacrificados.

RESULTADOS:

A parasitemia foi progressiva em todos os grupos inoculados. Ao final do experimento, os animais com esplenectomia parcial apresentaram parasitemia superior, porém próximos as dos não esplenectomizados, enquanto que os asplênicos apresentaram diferença superior a 100 por cento. Os níveis de IgM e IgG totais séricos não foram alterados significativamente com a remoção parcial ou total esplênica.

CONCLUSÃO:

As técnicas conservadoras no trauma esplênico são possíveis e necessárias. A importância do remanescente esplênico no clearence das hemácias infectadas pelo Plasmodium berghei demonstrou ser eficiente, de modo a evitar sérias complicações decorrentes da malária em camundongos.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Plasmodium berghei / Splenectomy / Parasitemia / Malaria Type of study: Diagnostic study / Observational study / Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2009 Type: Article Affiliation country: Brazil Institution/Affiliation country: UFAM/BR

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Full text: Available Index: LILACS (Americas) Main subject: Plasmodium berghei / Splenectomy / Parasitemia / Malaria Type of study: Diagnostic study / Observational study / Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2009 Type: Article Affiliation country: Brazil Institution/Affiliation country: UFAM/BR