Papel da imunofenotipagem por citometria de fluxo no diagnóstico diferencial das pancitopenias e das linfocitoses / The role of flow cytometer-based immunophenotyping in the differential diagnosis of pancytopenia and of lymphocytosis
Rev. bras. hematol. hemoter
;
31(5): 367-374, 2009. tab
Article
in Portuguese
| LILACS
| ID: lil-533601
RESUMO
A imunofenotipagem por citometria de fluxo (CMF) é atualmente uma ferramenta indispensável para o diagnóstico hematopatológico. Nos últimos anos muitos progressos foram alcançados em instrumentação, novos anticorpos e fluorocromos e programas de análise. Consequentemente, houve um grande avanço no conhecimento da patogênese das neoplasias hematológicas e novos marcadores diagnósticos e prognósticos foram descritos. Revisamos aqui a contribuição destas novas técnicas no diagnóstico diferencial de pacientes com bi- ou pancitopenia e linfocitose. São apresentados os achados mais frequentes e as dificuldades na interpretação dos resultados. Além disto, a importância do uso concomitante de um conjunto de outras técnicas diagnósticas é demonstrada.
ABSTRACT
The use of flow cytometry for immunophenotyping is currently an essential tool in the diagnosis of hematological abnormalities. In recent years, new equipment, antibodies, fluorochromes and computer programs have become available. As a result, a better understanding of the pathogeneses of hematological malignancies has emerged and new markers with diagnostic and prognostic relevance have been described. Here we review how this new technology may contribute to the differential diagnosis of patients with bi- or pancytopenia and of lymphocytosis. The common findings as well as the difficulties in interpreting the results obtained by flow cytometry will be discussed. The importance of the concomitant analyses by different methods is also demonstrated.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Pancytopenia
/
Immunophenotyping
/
Diagnosis, Differential
/
Flow Cytometry
/
Lymphocytosis
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
Portuguese
Journal:
Rev. bras. hematol. hemoter
Journal subject:
Hematology
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Hemocentro de Ribeirão Preto/BR
/
USP/BR
Similar
MEDLINE
...
LILACS
LIS