Aislamiento de Helicobacter pylori en sarro dental de pacientes con gastritis del policlinico Angamos / Isolation of Helicobacter pylori in dental plaque of patients with gastritis of the polyclinical

RESUMEN

Objetivo: Detectar la presencia de Helicobacter pylori en sarro dentario de pacientes con gastritis del Hospital Angamos ESSALUD diagnosticados por biopsia. MATERIAL Y METODOS: Tipo de estudio Observacional analítico. Población; 115 pacientes programados para endoscopia del servicio de gastroenterología de dicho hospital. La metodología empleada para la biopsia del antro y fondo del estomágo fue a través de gastroscopio. Recolectada la muestra se procesó para el diagnóstico histológico de gastritis y la búsqueda de Helicobacter pylori. La muestra de sarro dentario se obtuvo por raspado de la zona supragingival y transportada en medio de conservación para el cultivo de la bacteria en medio selectivo e incubado en microaerofilia por 5 a 10 días, se hizo coloración de las colonias sospechosas y compararon con una cepa patrón, la prueba de ureasa y oxidasa confirmaron el diagnóstico. RESULTADOS: Se hallaron 66 casos de gastritis asociados a H. p. por biopsia. En 24 casos también se logró aislar H.p. en sarro dentario. El otro grupo de 49 casos de gastritis sin H.p. en 4 hubo aislamiento de la bacteria en sarro dentario pero si en 4 casos. CONCLUSION: Los resultados indican una relación directa con los casos de gastritis y la positividad en el sarro dentario de Helicobacter pylori lo cual indicaría una relación de los pacientes de estos casos como reservorio de dicha bacteria, en comparación con los casos en la cual no hay la presencia de H.p. en los casos de gastritis sin Helicobacter, excepto en cuatro casos.

ABSTRACT

Objective: Detect the presence of (Helicobacter pylori) in dental plaque among patients with gastritis diagnosed by biopsy at Angamos Surquillo Hospital.MATERIALS AND METHODS: Type of study Analytical Observation. Population 115 patients scheduled for endoscopy at the Gastroenterological Department of said hospital. The methodology employed for the biopsy of the antrum and fundus of the stomach was by means of a gastroscope. Once the sample was collected it was processed and stained with hematoxilin-eosin for the histological diagnosis of gastritis by observation of Helicobacter pylori. Dental plaque samples were obtained by scraping the supragingival area and were transported in a conservation medium for culture of the bacteria in a selective medium and incubated in microaerophilia for 5 to 10 days; suspected colonies were stained and compared to a patron strain. Urease and oxidase tests confirmed the diagnosis.RESULTS: In total, 66 cases of gastritis associated with H.p. were found by biopsy. In 24 of these cases, H.p from dental plaque were also isolated. In another group of 49 cases of gastritis not associated with H.p., no H.p. was found in the dental plaque of 45 of these cases while H.p. was isolated from dental plaque in 4 cases. CONCLUSION: The results show a direct relation between the cases of gastritis andthe finding of Helicobacter pylori in dental plaque, which would indicate a relation between the patients and the bacteria in these cases, as they act as a reservoir for said bacteria; as compared with the cases in which there is no H.p presence in gastritis without Helicobacter, except in four cases. The cases of gastritis with Helicobacter pylori and negative dental plaque are notsignificant, in these cases the bacteria entered with the food.