Evaluation of HA negatively charged membranes in the recovery of human adenoviruses and hepatitis A virus in different water matrices
Mem. Inst. Oswaldo Cruz
;
104(7): 970-974, Nov. 2009. tab
Article
in English
| LILACS
| ID: lil-534160
ABSTRACT
Human adenoviruses (HAdV) and hepatitis A virus (HAV) are shed in the faeces and consequently may be present in environmental waters, resulting in an increase in pathogen concentration that can affect water quality and human health. The aim of this study was to evaluate an adsorption-elution method which utilizes negatively charged membrane HA to determine the efficient recovery of HAdV and HAV from different water matrices and to combine this procedure with a qualitative molecular method (nested RT-PCR and nested PCR). The best efficiency recovery was achieved in distilled water and treated wastewater effluent (100 percent) for both viruses and in recreational lagoon water for HAV (100 percent). The efficiency recovery was 10 percent for HAdV and HAV in seawater and 10 percent for HAdV in lagoon water. The viral detection limit by nested PCR for HAV in water samples ranged between 20-0.2 FFU/mL and 250 and 25 TCID50/mL for HAdV. In conclusion, these results suggest that the HA negatively charged membranes vary their efficiency for recovery of viral concentration depending upon the types of both enteric viruses and water matrices.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Water Microbiology
/
Adenoviruses, Human
/
Environmental Monitoring
/
Hepatitis A Virus, Human
/
Filtration
Type of study:
Evaluation studies
/
Qualitative research
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Departamento de Microbiologia e Parasitologia/BR
/
Universidade Federal de Santa Catarina/BR
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