Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
Mem. Inst. Oswaldo Cruz
;
104(7): 998-1002, Nov. 2009. ilus
Article
in English
| LILACS
| ID: lil-534165
ABSTRACT
Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Ribosomal Proteins
/
Protozoan Proteins
/
Babesia bovis
/
Antigens, Protozoan
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Embrapa Gado de Corte/BR
/
Embrapa Pecuária Sul/BR
/
Universidade Federal Rural de Pernambuco/BR
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