Tissue expression and subcellular localization of Mipu1, a novel myocardial ischemia-related gene
Braz. j. med. biol. res
; 43(1): 43-51, Jan. 2010. ilus, tab
Article
in En
| LILACS
| ID: lil-535635
Responsible library:
BR1.1
ABSTRACT
Myocardial ischemic preconditioning up-regulated protein 1 (Mipu1), a novel zinc finger protein, was originally cloned using bioinformatic analysis and 5' RACE technology of rat heart after a transient myocardial ischemia/reperfusion procedure in our laboratory. In order to investigate the functions of Mipu1, the recombinant prokaryotic expression vector pQE31-Mipu1 was constructed and transformed into Escherichia coli M15(pREP4), and Mipu1-6His fusion protein was expressed and purified. The identity of the purified protein was confirmed by mass spectrometry. The molecular mass of the Mipu1 protein was 70.03779 kDa. The fusion protein was intracutaneously injected to immunize New Zealand rabbits to produce a polyclonal antibody. The antibody titer was approximately 116,000. The antibody was tested by Western blotting for specificity and sensitivity. Using the antibody, it was found that Mipu1 was highly expressed in the heart and brain of rats and was localized in the nucleus of H9c2 myogenic cells. The present study lays the foundation for further study of the biological functions of Mipu1.
Key words
Full text:
1
Index:
LILACS
Main subject:
Repressor Proteins
/
Brain Chemistry
/
Nuclear Proteins
/
Myocardial Ischemia
/
Antibodies, Monoclonal
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Myocardium
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Braz. j. med. biol. res
Journal subject:
BIOLOGIA
/
MEDICINA
Year:
2010
Type:
Article
/
Project document