Análisis retrospectivo del rendimiento de Amplicor-PCR® para la detección de Mycobacterium tuberculosis en muestras respiratorias y no respiratorias con baciloscopia negativa: A retrospective analysis / Assessment of the Amplicor PCR® for the detection of Mycobacterium tuberculosis in smear negative respiratory and non respiratory specimens
Rev. chil. infectol
;
26(6): 495-498, dic. 2009. tab
Article
in Spanish
| LILACS
| ID: lil-536827
ABSTRACT
Background:
Commercial polymerase chain reaction (PCR) kits are widely accepted for analysis of smear positive respiratory specimens, but the sensitivity is variable for smear negative ones.Objective:
To assess the PCR method usefulness in smear negative respiratory and non respiratory specimens.Methods:
We compared the PCR results (AMPLICOR MTB test, Roche) of 235 specimens subjected to culture in Loewenstein-Jensen agar (as the gold standard).Results:
181 (76 percent) were respiratory and 54 (24 percent) extra-respiratory specimens. The sensitivity was 88 percent) and 50 percent>, respectively, specificity and PPV was 100 percent> in both cases. NPV was 99.4 percent> in respiratory specimens and 96.1 percent in non-respiratory specimens.Conclusions:
The good performance of this PCR in smear negative respiratory specimens allows the clinician to take decisions based on the result of this exam. In extra-respiratory specimens the contribution is important only when the PCR result is positive.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tuberculosis
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Limits:
Humans
Language:
Spanish
Journal:
Rev. chil. infectol
Journal subject:
Communicable Diseases
Year:
2009
Type:
Article
Affiliation country:
Chile
Institution/Affiliation country:
Pontificia Universidad Católica de Chile/CL
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