Your browser doesn't support javascript.
loading
Influence of ingaalp laser (660nm) on the healing of skin wounds in diabetic rats / Influência do laser ingaalp (660nm) na cicatrização de feridas cutâneas em ratos diabéticos
Carvalho, Paulo de Tarso Camillo de; Silva, Iandara Schettert da; Reis, Filipe Abdalla dos; Perreira, Daniel Martins; Aydos, Ricardo Dutra.
  • Carvalho, Paulo de Tarso Camillo de; Federal University of Sergipe. Department of Physiotherapy. Aracaju. BR
  • Silva, Iandara Schettert da; UFMS. West Central Region. Postgraduate Program in Health and Development. Campo Grande. BR
  • Reis, Filipe Abdalla dos; UFMS. West Central Region. Postgraduate Program in Health and Development. Campo Grande. BR
  • Perreira, Daniel Martins; UNIDERP. Department of Physiotherapy. Campo Grande. BR
  • Aydos, Ricardo Dutra; UFMS. West Central Region. Postgraduate Program in Health and Development. Campo Grande. BR
Acta cir. bras ; 25(1): 71-79, jan.-fev. 2010. ilus
Article in English | LILACS | ID: lil-537125
ABSTRACT

PURPOSE:

To determine the influence of low-power laser (660 nm) on the collagen percentage and macrophages in skin wounds in diabetic rats.

METHODS:

30 male Wistar rats were used, distributed in two groups laser treated diabetic (n= 15); untreated diabetic (n = 15). The diabetes was induced by intravenous injection of alloxan into the dorsal vein of the penis, at a rate of 0.1 ml of solution per 100 g of body weight. A wound was made on the back of all the animals. Groups 2 were treated with Aluminium Gallium Indium Phosphide - InGaAlP type diode laser (Photon Laser III DMC®) with a continuous output power of 100 mW and wavelength (?) of 660 nm (4 J/cm²) for 24 s. five animal from each group was sacrificed on the 3rd, 7th and 14th days after wounding. Samples were taken, embedded in paraffin, stained with hematoxylin-eosin, Masson's trichrome, and immunohistochemical macrophage. morphometrically analyzed using the Image Pro Plus 4.5 software. The percentages of collagen fibers and macrophages were determined from the samples from the euthanasia animals.

RESULTS:

The data were treated statistically using analysis of variance (ANOVA) and the Post-hocTukey test. The significance level was set at 0.05 or 5 percent.

CONCLUSION:

The low-power laser (660 nm) was shown to be capable of influencing the collagen percentage in skin wounds by increasing the mean quantity of collagen fibers and macrophages.
RESUMO

OBJETIVO:

Determinar a influência do diodo laser InGaAlP (660 nm) sobre o percentual de colágeno e macrófagos em feridas cutâneas de ratos diabéticos.

MÉTODOS:

Para tanto 30 ratos machos Wistar foram distribuídos em dois grupos diabético tratado com laser (n=15), diabético não tratado (n = 15). O diabetes foi induzido por injeção intravenosa de aloxana na veia dorsal do pênis, a uma taxa de 0,1 ml de solução por 100 g de peso corporal. A lesão foi confeccionada no dorso de todos os animais utilizando um punch de 8mm. 2 grupos foram tratados com o diodo laser arsenieto de gálio e alumínio dopado com, fosforeto de índio - InGaAlP (tipo Photon Laser III DMC®) com uma potência contínua de 100 mW e comprimento de onda (?) de 660 nm (4 J/cm²) por 24 s. cinco animais de cada grupo foi sacrificado no 3, 7 e 14 dias após a lesão. As amostras obtidas foram emblocadas em parafina, coradas com hematoxilina-eosina, tricrômico de Masson, e para a analise dos macrófagos foi realizado um imunohistoquímico. Os resultados foram avaliados morfometricamente pelo programa Image Pro Plus 4.5.

RESULTADOS:

Os dados foram tratados estatisticamente pela análise de variância (ANOVA) e pós-teste de Tukey. O nível de significância foi fixado em 0,05 ou 5 por cento.

CONCLUSÃO:

O laser de baixa potência (660 nm) mostrou-se capaz de influenciar o percentual de colágeno em feridas cutâneas, aumentando a quantidade média de fibras colágenas e macrófagos.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Skin / Wound Healing / Collagen / Low-Level Light Therapy / Diabetes Mellitus, Experimental / Macrophages Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Sergipe/BR / UFMS/BR / UNIDERP/BR

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: LILACS (Americas) Main subject: Skin / Wound Healing / Collagen / Low-Level Light Therapy / Diabetes Mellitus, Experimental / Macrophages Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal University of Sergipe/BR / UFMS/BR / UNIDERP/BR