Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
Mem. Inst. Oswaldo Cruz
;
104(8): 1055-1062, Dec. 2009. ilus
Article
in English
| LILACS
| ID: lil-538164
ABSTRACT
Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
Proline
/
Enzyme Inhibitors
/
Amino Acid Isomerases
/
Host-Parasite Interactions
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Type:
Article
Affiliation country:
Brazil
/
France
Institution/Affiliation country:
Institut Pasteur/FR
/
Instituto Oswaldo Cruz-Fiocruz/BR
/
Laboratório de Genômica Funcional e Bioinformática/BR
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