Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples
Mem. Inst. Oswaldo Cruz
;
104(8): 1194-1196, Dec. 2009. tab
Article
in English
| LILACS
| ID: lil-538184
ABSTRACT
A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Schistosomiasis mansoni
/
Polymerase Chain Reaction
/
DNA, Protozoan
/
Feces
Type of study:
Diagnostic study
/
Evaluation studies
Limits:
Aged80
/
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
/
Laboratório de Pesquisas Clínicas/BR
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