Comparison of immunoperoxidase and immunofluorescence assays for pp65 cytomegalovirus antigen in immunocompromised patients
Braz. j. infect. dis
;
13(2): 142-146, Apr. 2009. tab, graf
Article
in English
| LILACS
| ID: lil-538221
ABSTRACT
We compared the pp65 antigen detection by an in house method (immunoperoxidase assay) and by a commercial kit (immunofluorescence assay) available for cytomegalovirus infection diagnosis in immunocompromised patients. Sixty-four blood samples were analyzed in duplicate for both techniques. Eight-six percent of the samples had concordant qualitative results. The discordant results occurred more frequently in samples with low quantity of positive cells. There were no significant differences with qualitative and quantitative results of the methods.
Full text:
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Index:
LILACS (Americas)
Main subject:
Phosphoproteins
/
Viral Matrix Proteins
/
Immunocompromised Host
/
Cytomegalovirus Infections
/
Cytomegalovirus
Type of study:
Diagnostic study
/
Qualitative research
Limits:
Adult
/
Female
/
Humans
/
Male
/
Pregnancy
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Paraná/BR
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