Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
Mem. Inst. Oswaldo Cruz
;
105(1): 62-65, Feb. 2010. ilus
Article
in English
| LILACS
| ID: lil-539297
ABSTRACT
This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic DNA (RAPD) analysis. The RAPD technique can be helpful for distinguishing between clinical isolates. Different random primers were used to characterise these clinical isolates. The RAPD patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28 percent genetic variability in this fungal species, opening new possibilities for clinical studies of P. brasiliensis. Based on these results and preliminary clinical findings, we suggest that different genotypes of P. brasiliensis might infect the same patient, inducing the active form of the disease.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Paracoccidioides
/
Paracoccidioidomycosis
/
DNA, Fungal
Limits:
Humans
/
Male
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2010
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Mato Grosso/BR
/
Universidade Federal de São Paulo/BR
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