Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre
Mem. Inst. Oswaldo Cruz
;
105(1): 109-112, Feb. 2010. tab
Article
in English
| LILACS
| ID: lil-539304
ABSTRACT
The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3 percent sensitivity, 93.3 percent specificity, a 99.2 percent positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.
Full text:
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Index:
LILACS (Americas)
Main subject:
Polymerase Chain Reaction
/
DNA, Protozoan
/
Leishmaniasis, Cutaneous
/
Leishmania
Type of study:
Diagnostic study
/
Evaluation studies
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2010
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
/
Instituto de Pesquisa Clínica Evandro Chagas/BR
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