Comparison of HPV genotyping by type-specific PCR and sequencing
Mem. Inst. Oswaldo Cruz
;
105(1): 73-78, Feb. 2010. tab, ilus
Article
in English
| LILACS
| ID: lil-539308
ABSTRACT
Human papillomavirus (HPV) infection is the most common sexually transmitted disease worldwide and there is a strong link between certain high-risk viral types and cervical carcinogenesis. Although there are several typing methods, it is still unclear which test is the best. This study compared the effectiveness of type-specific PCR (TS-PCR) and sequencing, with a focus on their clinical application. A total of 260 cervical samples from HPV-positive patients were tested for types 6, 11, 16, 18, 31, 33 and 35 using TS-PCR and sequencing. The genotype was identified in 36 percent of cases by TS-PCR and in 75 percent by sequencing. Sequencing was four times more likely to identify the viral type in positive samples than TS-PCR (p = 0.00). Despite being more effective for virus genotyping, sequencing was unable to identify viral types in multiple infections. Combining both techniques resulted in highly sensitive detection (87 percent of cases), showing that they are complementary methods. HPV genotyping is an important step in HPV management, helping to identify patients with a higher risk of developing cervical cancer and contributing to the development of type-specific vaccines.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Papillomaviridae
/
DNA, Viral
/
Polymerase Chain Reaction
/
Papillomavirus Infections
/
Genotype
Limits:
Female
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2010
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro de Treinamento e Referência em Doenças Infecciosas e Parasitárias Orestes Diniz/BR
/
Universidade Federal de Minas Gerais/BR
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