A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation
Biocell
;
32(1): 1-8, Apr. 2008. ilus
Article
in English
| LILACS
| ID: lil-541117
ABSTRACT
Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Central Nervous System
/
Actins
/
Eosine Yellowish-(YS)
/
Photooxidation
Limits:
Animals
/
Humans
Language:
English
Journal:
Biocell
Journal subject:
Clulas
Year:
2008
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad de Buenos Aires/AR
Similar
MEDLINE
...
LILACS
LIS