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Parâmetros cinéticos de linhagens de levedura isoladas de alambiques mineiros / Kinetic parameters of yeasts strains isolated from cachaça distilleries in Minas Gerais/Brazil
Stroppa, Cibele Tosin; Alves, José Guilherme Lembi Ferreira; Figueiredo, Ana Luíza França de.
  • Stroppa, Cibele Tosin; s.af
  • Alves, José Guilherme Lembi Ferreira; Universidade Federal de Lavras. Departamento de Ciência dos Alimentos. Lavras. BR
  • Figueiredo, Ana Luíza França de; s.af
Ciênc. agrotec., (Impr.) ; 33(spe): 1978-1983, 2009. tab, ilus
Article in Portuguese | LILACS | ID: lil-542355
RESUMO
Foram isoladas leveduras prevalescentes de dois alambiques, em Betim e Rio Manso/MG, por meio de plaqueamento (WLN) e caracterizadas segundo a macromorfologia de colônias, após 8 dias de incubação a 32ºC. A amostra de Rio Manso apresentou 4 linhagens dominantes, com concentrações entre 2,0 x 10(7) e 4,0 x 10(5) UFC/mL. No alambique de Betim foram isoladas três linhagens dominantes, com contagens entre 1,3 x 10(8) e 2,0 x 10(4) UFC/mL. Foram determinados a velocidade específica máxima de crescimento (µmax) e o fator de conversão de substrato em biomassa (Yx/s) para dois isolados. Os valores de μmax foram 0,24 e 0,4 h-1, sendo que a linhagem RM01 apresentou maior valor. Para Yx/s, os resultados obtidos pelas linhagens RM01 e CV01 foram 0,179 e 0,185 g.g-1, respectivamente. Verificou-se uma semelhança nos resultados entre as linhagens RM01 e CV01. Concluiu-se que, do ponto de vista de μmax e de Yx/s, a melhor linhagem para produção de cachaça é a RM01.
ABSTRACT
Predominant yeasts of two cachaça distilleries were isolated in the cities of Betim and Rio Manso, in the State of Minas Gerais and characterized using WLN agar, according to colony morphology, after 8 days of imcubation at 32ºC. The sample from Rio Manso presented 4 dominant yeasts with concentrations between 2.0 x 10(7) and 4.0 x 10(5) CFU/mL. At Betim distillery, three dominant yeasts were isolated with cell density between 1.3 x 10(8) and 2.0 x 10(4) CFU/mL. The values of the maximum specific velocity for growth (µmax) and the conversion factor for substrate into cells (Yx/s) were determined for two isolates. The μmax values were 0.24 and 0.4 h-1, being RM01 strain the one with the highest value. Regarding to Yx/s, the results obtained by strains RM01 and CV01 were 0.179 and 0.185 g.g-1, respectively. Resemblances of results were observed between strains RM01 and CV01. It was concluded that, as far as μmax and Yx/s are concerned, the best strain to produce cachaça is RM01.

Full text: Available Index: LILACS (Americas) Country/Region as subject: South America / Brazil Language: Portuguese Journal: Ciênc. agrotec., (Impr.) Journal subject: Biology / Biotechnology / Nutritional Sciences / VETERINARIA Year: 2009 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Lavras/BR

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Full text: Available Index: LILACS (Americas) Country/Region as subject: South America / Brazil Language: Portuguese Journal: Ciênc. agrotec., (Impr.) Journal subject: Biology / Biotechnology / Nutritional Sciences / VETERINARIA Year: 2009 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Lavras/BR