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Genetic polymorphism of Streptococcus mutans strains associated with incomplete caries removal
Duque, Cristiane; Negrini, Thais de Cássia; Sacono, Nancy Tomoko; Boriollo, Marcelo Fabiano Gomes; Hofling, José Francisco; Hebling, Josimeri; Spolidorio, Denise Madalena Palomari.
  • Duque, Cristiane; Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba. Department of Microbiology and Immunology. Piracicaba. BR
  • Negrini, Thais de Cássia; Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Department of Clinical Analysis. Araraquara. BR
  • Sacono, Nancy Tomoko; Universidade Estadual Paulista. Faculdade de Odontologia de Araraquara. Department of Orthodontics and Pediatric Dentistry. Araraquara. BR
  • Boriollo, Marcelo Fabiano Gomes; Universidade José do Rosário Vellano. Department of Microbiology. Alfenas. BR
  • Hofling, José Francisco; Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba. Department of Microbiology and Immunology. Piracicaba. BR
  • Hebling, Josimeri; Universidade Estadual Paulista. Faculdade de Odontologia de Araraquara. Department of Orthodontics and Pediatric Dentistry. Araraquara. BR
  • Spolidorio, Denise Madalena Palomari; Universidade Estadual Paulista. Faculdade de Odontologia de Araraquara. Department of Physiology and Pathology. Araraquara. BR
Braz. j. oral sci ; 8(1): 2-8, Jan.-Mar. 2009. ilus, tab
Article in English | LILACS, BBO | ID: lil-542849
ABSTRACT

Aim:

Despite the antibacterial properties of dental materials, the survival of residual bacteria under restorations has been demonstrated after incomplete caries removal. The aim of this study was to evaluate the genetic polymorphism of Streptococcus mutans strains isolated from deep dentinal lesions before and three months after incomplete caries removal.

Methods:

Samples of carious dentin were collected from 33 primary and/or permanent molars before and after indirect pulp treatment and processed for microbiological isolation of mutans streptococci (MS). After three months of the dental treatment, positive cultures for MS were detected in only ten of these teeth. DNA of MS isolates were obtained and subjected to polymerase chain reaction (PCR) for identification of S mutans. The arbitrary primed-PCR method (primer OPA-13) was used to detect the genetic polymorphism of S. mutans strains.

Results:

Identical or highly related S. mutans genotypes were observed in each tooth, regardless of the collect. Considering each tooth separately, a maximum of nine genotypic patterns were found in each tooth from all the collects. In addition, at least one genotypic pattern was repeated in the three collects. Genetic diversity was observed among the S. mutans isolates, obtained from different teeth after three months of the dental treatment.

Conclusions:

The persistence of identical genotypic patterns and the genetic similarity among the isolates, from the same tooth in distinct collects, showed the resistance of some S. mutans strains after incomplete caries removal treatment.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Polymorphism, Genetic / Streptococcus mutans / Dental Caries Type of study: Risk factors Limits: Female / Humans / Male Language: English Journal: Braz. j. oral sci Journal subject: Dentistry Year: 2009 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Estadual de Campinas/BR / Universidade José do Rosário Vellano/BR

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Full text: Available Index: LILACS (Americas) Main subject: Polymorphism, Genetic / Streptococcus mutans / Dental Caries Type of study: Risk factors Limits: Female / Humans / Male Language: English Journal: Braz. j. oral sci Journal subject: Dentistry Year: 2009 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade Estadual de Campinas/BR / Universidade José do Rosário Vellano/BR