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Histological study of fresh versus frozen semitendinous muscle tendon allografts
Bitar, Alexandre Carneiro; Santos, Luiz Augusto Ubirajara; Croci, Alberto Tesconi; Pereira, João Alberto Ramos Maradei; França Bisneto, Edgard N; Giovani, Arlete Mazzini Miranda; Oliveira, Claudia Regina G. C. M.
  • Bitar, Alexandre Carneiro; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clinicas. Institute of Orthopedics and Traumatology. São Paulo. BR
  • Santos, Luiz Augusto Ubirajara; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clinicas. Institute of Orthopedics and Traumatology. São Paulo. BR
  • Croci, Alberto Tesconi; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clinicas. Institute of Orthopedics and Traumatology. São Paulo. BR
  • Pereira, João Alberto Ramos Maradei; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clinicas. Institute of Orthopedics and Traumatology. São Paulo. BR
  • França Bisneto, Edgard N; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clinicas. Institute of Orthopedics and Traumatology. São Paulo. BR
  • Giovani, Arlete Mazzini Miranda; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clinicas. Institute of Orthopedics and Traumatology. São Paulo. BR
  • Oliveira, Claudia Regina G. C. M; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clinicas. Institute of Orthopedics and Traumatology. São Paulo. BR
Clinics ; 65(3): 297-303, 2010. tab, ilus
Article in English | LILACS | ID: lil-544009
ABSTRACT
OBJECTIVE: The purpose of this study was to histologically analyze allografts from cadaveric semitendinous muscle after cryopreservation at -80°C in comparison to a control group kept at only -4°C to test the hypothesis that the histological characteristics of the tissue are maintained when the tendons are kept at lower temperatures. METHODS: In a tissue bank, 10 semitendinous tendons from 10 cadavers were frozen at -80ºC as a storage method for tissue preservation. They were kept frozen for 40 days, and then a histological study was carried out. Another 10 tendon samples were analyzed while still "fresh". RESULTS: There was no histological difference between the fresh and frozen samples in relation to seven variables. CONCLUSIONS: Semitendinous muscle tendon allografts can be submitted to cryopreservation at -80ºC without suffering histological modifications.
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Full text: Available Index: LILACS (Americas) Main subject: Tendons / Cryopreservation / Muscle, Skeletal Limits: Adult / Female / Humans / Male Language: English Journal: Clinics Journal subject: Medicine Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Tendons / Cryopreservation / Muscle, Skeletal Limits: Adult / Female / Humans / Male Language: English Journal: Clinics Journal subject: Medicine Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR