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Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
Miura, Flávio Key; Alves, Maria Jose Ferreira; Rocha, Mussya Cisotto; Silva, Roseli da; Oba-Shinjo, Sueli Mieko; Marie, Suely Kazue Nagahashi.
  • Miura, Flávio Key; Universidade de São Paulo. Faculdade de Medicina. Laboratory of Molecular and Cellular Biology. São Paulo. BR
  • Alves, Maria Jose Ferreira; Universidade de São Paulo. Faculdade de Medicina. Laboratory of Molecular and Cellular Biology. São Paulo. BR
  • Rocha, Mussya Cisotto; Universidade de São Paulo. Faculdade de Medicina. Laboratory of Molecular and Cellular Biology. São Paulo. BR
  • Silva, Roseli da; Universidade de São Paulo. Faculdade de Medicina. Laboratory of Molecular and Cellular Biology. São Paulo. BR
  • Oba-Shinjo, Sueli Mieko; Universidade de São Paulo. Faculdade de Medicina. Laboratory of Molecular and Cellular Biology. São Paulo. BR
  • Marie, Suely Kazue Nagahashi; Universidade de São Paulo. Faculdade de Medicina. Laboratory of Molecular and Cellular Biology. São Paulo. BR
Clinics ; 65(3): 305-309, 2010. ilus
Article in English | LILACS | ID: lil-544010
ABSTRACT

INTRODUCTION:

Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60 percent of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential.

OBJECTIVE:

To develop an experimental malignant astrocytoma model with the characteristics of the human tumor.

METHOD:

Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats.

RESULTS:

All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed.

CONCLUSION:

A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal's immune system.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Astrocytoma / Brain Neoplasms / Immunocompromised Host / Glioblastoma Type of study: Prognostic study Limits: Animals / Female / Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2010 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Astrocytoma / Brain Neoplasms / Immunocompromised Host / Glioblastoma Type of study: Prognostic study Limits: Animals / Female / Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2010 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR