Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells
Mem. Inst. Oswaldo Cruz
;
105(2): 132-136, Mar. 2010. ilus
Article
in English
| LILACS
| ID: lil-544617
ABSTRACT
In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Rhodnius
/
Salivary Glands
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2010
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Ezequiel Dias/BR
/
Universidade Federal de Minas Gerais/BR
Similar
MEDLINE
...
LILACS
LIS