Evaluation of buffy coat 16S rRNA PCR, buffy coat culture and whole blood PCR for detection of bacteraemia
Mem. Inst. Oswaldo Cruz
;
105(2): 117-122, Mar. 2010. ilus
Article
in English
| LILACS
| ID: lil-544633
ABSTRACT
The use of Gram type-specific PCR on buffy coat from clinical specimens for the detection of bacteraemia was evaluated for the first time using whole blood culture as the gold standard. In addition, the established buffy coat culture and whole blood PCR were also compared. Gram-positive bacteria belonging to six species and Gram-negative bacteria from 10 species were isolated and identified by culture and detected using broad-range 16S rDNA primers and Gram-specific primers. Data from the three methods all conferred very high sensitivity, specificity, positive and negative predictive values when compared to whole blood culture. The Kappa coefficients of agreement were 0.9819 (buffy coat PCR), 0.9458 (whole blood PCR) and 1.0 (buffy coat culture), which establishes their validity as alternative methods to routine blood culture in detecting bacteraemia. In addition, results showed that there was a direct correlation of WBC counts greater than 12,000 cells per mm³ to the occurrence of bacteraemia as detected by the four methods (p < 0.05).
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacteremia
/
Gram-Negative Bacteria
/
Gram-Positive Bacteria
/
Leukocytes
Type of study:
Diagnostic study
/
Evaluation studies
/
Prognostic study
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2010
Type:
Article
Affiliation country:
Pakistan
/
Philippines
Institution/Affiliation country:
De La Salle University/PH
/
Makati Medical Center/PH
/
Ospital ng Makati/PH
Similar
MEDLINE
...
LILACS
LIS