Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
Braz. j. microbiol
; 41(2): 278-282, Apr.-June 2010. tab, ilus
Article
in En
| LILACS
| ID: lil-545329
Responsible library:
BR32.1
ABSTRACT
The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1 percent) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8 percent of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR.
Key words
Full text:
1
Index:
LILACS
Main subject:
Patients
/
Pseudomonadaceae
/
Beta-Lactamases
/
Drug Resistance, Microbial
/
Base Sequence
/
Polymerase Chain Reaction
/
Enterobacteriaceae
/
Enterobacteriaceae Infections
/
Clinical Enzyme Tests
Type of study:
Diagnostic_studies
/
Prevalence_studies
/
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2010
Type:
Article