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Detection of mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
Figueiredo, Eduardo Eustáquio de Souza; Carvalho, Ricardo Cezar Tavares; Silvestre, Flávia Galindo; Lilenbaum, Walter; Fonseca, Leila Sousa; Silva, Joab Trajano; Paschoalin, Vânia Margaret Flosi.
  • Figueiredo, Eduardo Eustáquio de Souza; Universidade Federal do Rio de Janeiro. Rio de Janeiro. BR
  • Carvalho, Ricardo Cezar Tavares; Universidade de Cuiabá. Cuiabá. BR
  • Silvestre, Flávia Galindo; Universidade de Cuiabá. Cuiabá. BR
  • Lilenbaum, Walter; Universidade Federal Fluminense. Niterói. BR
  • Fonseca, Leila Sousa; Universidade Federal do Rio de Janeiro. Rio de Janeiro. BR
  • Silva, Joab Trajano; Universidade Federal do Rio de Janeiro. Rio de Janeiro. BR
  • Paschoalin, Vânia Margaret Flosi; Universidade Federal do Rio de Janeiro. Rio de Janeiro. BR
Braz. j. microbiol ; 41(2): 386-390, Apr.-June 2010. ilus
Article in English | LILACS | ID: lil-545347
ABSTRACT
Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9 percent). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
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Full text: Available Index: LILACS (Americas) Main subject: Tuberculosis, Bovine / In Vitro Techniques / DNA / Polymerase Chain Reaction / Mycobacterium bovis / Mycobacterium Infections Type of study: Diagnostic study Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal Fluminense/BR / Universidade Federal do Rio de Janeiro/BR / Universidade de Cuiabá/BR

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Full text: Available Index: LILACS (Americas) Main subject: Tuberculosis, Bovine / In Vitro Techniques / DNA / Polymerase Chain Reaction / Mycobacterium bovis / Mycobacterium Infections Type of study: Diagnostic study Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal Fluminense/BR / Universidade Federal do Rio de Janeiro/BR / Universidade de Cuiabá/BR