Detection of mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
Braz. j. microbiol
;
41(2): 386-390, Apr.-June 2010. ilus
Article
in English
| LILACS
| ID: lil-545347
ABSTRACT
Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9 percent). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tuberculosis, Bovine
/
In Vitro Techniques
/
DNA
/
Polymerase Chain Reaction
/
Mycobacterium bovis
/
Mycobacterium Infections
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2010
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal Fluminense/BR
/
Universidade Federal do Rio de Janeiro/BR
/
Universidade de Cuiabá/BR
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