Identificação de GRP75 como um romance proteína PreS1 usando uma estratégia proteômica / Identification of GRP75 as a novel pres1 binding protein protein using a proteomics strategy
Braz. j. microbiol
;
41(2): 512-518, Apr.-June 2010. ilus, tab
Article
in English
| LILACS
| ID: lil-545363
RESUMO
A região PreS1 da proteína L é importante na adesão celular e, consequentemente, o vírus da hepatite B (HBV) infectividade. Para identificar novas proteínas que interagem PreS1 , realizamos Glutationa -S -transferase (GST) suspenso, electroforese bidimensional do gel ( 2-DE) e os testes de espectrometria de massa. proteínas Glucose- regulamentado (GRP ) 78 e 75 foram encontrados para vincular PreS1 . As interações entre PreS1 e GRP75 foram confirmados por uma experiência de co - imunoprecipitação . GRP78 e GRP75 pode desempenhar um papel importante na mediação HBV virion entrar em hepatócitos e regulação dobradura apropriada da proteína L , devido às suas funções críticas de enovelamento de proteínas e tráfico. A descoberta do romance PreS1 proteína enriquece nosso conhecimento sobre o mecanismo molecular da infecção pelo HBV.
ABSTRACT
The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV infection.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Mass Spectrometry
/
In Vitro Techniques
/
Hepatitis B virus
/
Polymerase Chain Reaction
/
Hepatocytes
/
Proteomics
/
Glutathione S-Transferase pi
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2010
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Institute de Microbiologia 2Jiangsu Provincial Centro de Doenças Prevenção e Controle/CN
/
Institute de Microbiologia/CN
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