Production and use of egg-yolk antibody for detection of canine parvovirus in feces
Rev. Ciênc. Méd. Biol. (Impr.)
;
7(3): 241-248, set.-dez. 2008. ilus, graf, tab
Article
in English
| LILACS, BBO
| ID: lil-545789
ABSTRACT
Canine parvovirus (CPV), cause an intestinal disease characterized by bloody diarrhea, is often fatal in puppies. The virus is transmitted by contact with infected dogs or their feces. The virus is very stable in the environment and may survive for several months in contaminated areas. The CPV attacks the rapidly diving cells of the bone marrow and the small intestine. Several laboratory tests have been developed and are available for specific viral diagnosis. Where facilities are available, rapid diagnosis can be made by electron microscopy (EM) of fecal material from cases with typical signs of disease. The virus also can be isolated in several feline and canine cell lines such as canine and feline kidney cells, but isolation is seldom used in practice since cell cultures are required and at least 1 week for results is required. Fecal hemagglutination-hemagglutination (HA-HI) tests have provided a simple and rapid method for detecting virus in fecal and tissue samples and are employed by several diagnostic laboratories, however the HA test is less sensitive than EM or enzyme-linked immunoassays (ELISA). In this work, the laying chickens are immunized with the canine parvovirus strain Cornell 780916-80 and the egg yolk antibody (IgY) isolated and characterized by indirect ELISA to detect canine parvovirus in feces.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Parvovirus, Canine
/
Diagnosis
/
Antibodies
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Rev. Ciênc. Méd. Biol. (Impr.)
Journal subject:
Biology
/
Medicine
Year:
2008
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Escola Técnica Estadual João Barcelos Martins/BR
/
UFES/BR
/
Universidade Estadual do Norte Fluminense Darcy Ribeiro/BR
/
Universidade Estadual do Norte Fluminense/BR
/
Universidade Salgado de Oliveira/BR
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