Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro
Mem. Inst. Oswaldo Cruz
;
105(3): 304-309, May 2010. graf, tab
Article
in English
| LILACS
| ID: lil-547301
ABSTRACT
The inhibitory effect of Lippia alba and Lippia citriodora essential oils on dengue virus serotypes replication in vitro was investigated. The cytotoxicity (CC50) was evaluated by the MTT assay and the mode of viral inhibitory effect was investigated with a plaque reduction assay. The virus was treated with the essential oil for 2 h at 37ºC before cell adsorption and experiments were conducted to evaluate inhibition of untreated-virus replication in the presence of oil. Antiviral activity was defined as the concentration of essential oil that caused 50 percent reduction of the virus plaque number (IC50). L. alba oil resulted in less cytotoxicity than L. citriodora oil (CC50 139.5 vs. 57.6 μg/mL). Virus plaque reduction for all four dengue serotypes was observed by treatment of the virus before adsorption on cell. The IC50 values for L. alba oil were between 0.4-32.6 μg/mL and between 1.9-33.7 μg/mL for L. citriodora oil. No viral inhibitory effect was observed by addition of the essential oil after virus adsorption. The inhibitory effect of the essential oil seems to cause direct virus inactivation before adsorption on host cell.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Antiviral Agents
/
Virus Replication
/
Plant Oils
/
Oils, Volatile
/
Lippia
/
Dengue Virus
Limits:
Animals
Country/Region as subject:
South America
/
Colombia
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2010
Type:
Article
/
Project document
Affiliation country:
Colombia
Institution/Affiliation country:
Centro de Investigaciones en Enfermedades Tropicales/CO
/
Universidad Industrial de Santande/CO
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