The role of GADD45A in resistance to oxidative stress-mediated cell death in human colon tumor cell lines
Appl. cancer res
;
29(4): 179-184, Oct.-Dec. 2009. tab, ilus
Article
in English
| LILACS, Inca
| ID: lil-547651
ABSTRACT
Objective:
GADD45A is a growth arrest-associated gene, directly involved in the maintenance of genomic stability. In fact, in the absence of this protein cells became sensitized to death by ultraviolet irradiation or cisplatin. However, this role with respect to cell damage triggered by Reactive Oxygen Species (ROS) is not well understood. Thus, in an attempt to evaluate its role in oxidative stress-induced apoptosis, we analyzed its expression after induction of ROS in human colon cell lines. Material andmethods:
Cell lines derived from human colon tumors (RKO-AS45-1 and HuTu-80) were treated with Menadione (MEN) and Hydrogen Peroxide (HP). Next, the expression of GADD45A was evaluated by semi-quantitative RT-PCR analysis.Results:
The results indicated that expression of this gene was associated with resistance to apoptosis. Additionally, cells with high expression of GADD45A were resistant to treatment with oxidative stress-inducing compounds.Conclusion:
In conclusion, we propose that the expression of the GADD45A gene can be used as a functional tool to predict cellular responses to antitumor treatments.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Colorectal Neoplasms
/
Reactive Oxygen Species
/
Apoptosis
/
Oxidative Stress
/
Hydrogen Peroxide
Language:
English
Journal:
Appl. cancer res
Journal subject:
Neoplasms
Year:
2009
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Ezequiel Dias/BR
/
Universidade de São Paulo/BR
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