Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)
Biol. Res
;
43(1): 63-74, 2010. ilus, graf
Article
in English
| LILACS
| ID: lil-548030
ABSTRACT
Astilbin (5,7,3,4-tetrahydroxy-2,3-dihydroflavonol-3-ß-o-rhamnoside), a flavonoid with a large range of biological activities, was isolated from Dimorphandra mollis, a shrub common to the Brazilian Cerrado. The purpose of this study is to verify the effects of astilbin on myeloperoxidase (MPO) and horseradish peroxidase (HRP), and its antioxidant activity against hypochlorous acid (HOCl) and total antioxidant activity (TAC) by the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+). Astilbin inhibited MPO and HRP activities in a concentration-dependent relationship and effectively scavenged HOCl. The TAC by ABTS+ of astilbin (IC50 ~ 20 mM) was higher than that of uric acid, which was used as a positive control. These data demonstrate that astilbin is a potent antioxidant and that it inhibits MPO and HRP activities efficiently.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plant Extracts
/
Free Radical Scavengers
/
Reactive Oxygen Species
/
Peroxidase
/
Flavonols
/
Fabaceae
/
Antioxidants
Limits:
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2010
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
São Paulo State University/BR
/
UFG/BR
/
UNICENTRO/BR
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