Characterization, purification and phylogenetic analysis of a cytolysin from the sea anemone Heteractis magnifica of the Indian Ocean
J. venom. anim. toxins incl. trop. dis
;
16(2): 223-240, 2010. ilus, graf, tab
Article
in English
| LILACS, VETINDEX
| ID: lil-548846
ABSTRACT
It is well established that sea anemones comprise a rich source of cytolytic toxins. The present study reports the isolation and characterization of a cytolysin obtained from the sea anemone Heteractis magnifica collected in the Andaman Islands of the Indian Ocean. The crude extract was screened for hemolytic activity by a blood agar plate method and a 6-mm zone of clearance was observed after incubation. The hemolytic property of the crude extract, tested by the microtiter plate method, revealed positive results at concentrations as low as 120 ng/mL. Furthermore, it was favored by alkaline pH and was stable up to 60°C. On the other hand, the hemolytic effect was abolished by the addition of human serum. Purification steps involved ammonium sulfate precipitation and subsequent desalting by dialysis, followed by anion- and cation-exchange chromatographies. The purified fractions displayed the presence of a 19-kDa cytolysin when analyzed by SDS-PAGE. The conserved region of the cytolysin (with 303 bp) was amplified by RT-PCR and was sequenced. The sequence showed maximum homology (97 percent) with the already reported cytolysins from other sea anemone species.(AU)
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Phylogeny
/
Sea Anemones
/
Cytotoxins
Limits:
Animals
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Year:
2010
Type:
Article
Institution/Affiliation country:
Dr. M. G. R University/IN
/
National Institute of Ocean Technology/IN
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