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Biosurfactant production by Rhodococcus erythropolis and its application to oil removal
Pacheco, Graziela Jardim; Ciapina, Elisa Mara Prioli; Gomes, Edelvio de Barros; Pereira Junior, Nei.
  • Pacheco, Graziela Jardim; Universidade Federal do Rio de Janeiro. Faculdade de Química. Centro de Tecnologia. Departamento de Engenharia Bioquímica. Rio de Janeiro. BR
  • Ciapina, Elisa Mara Prioli; Universidade Federal do Rio de Janeiro. Faculdade de Química. Centro de Tecnologia. Departamento de Engenharia Bioquímica. Rio de Janeiro. BR
  • Gomes, Edelvio de Barros; Universidade Federal do Rio de Janeiro. Faculdade de Química. Centro de Tecnologia. Departamento de Engenharia Bioquímica. Rio de Janeiro. BR
  • Pereira Junior, Nei; Universidade Federal do Rio de Janeiro. Faculdade de Química. Centro de Tecnologia. Departamento de Engenharia Bioquímica. Rio de Janeiro. BR
Braz. j. microbiol ; 41(3): 685-693, Oct. 2010. ilus, graf, tab
Article in English | LILACS | ID: lil-549410
ABSTRACT
The influence of different nutrients on biosurfactant production by Rhodococcus erythropolis was investigated. Increasing the concentration of phosphate buffer from 30 up through 150 mmol/L stimulated an increase in biosurfactant production, which reached a maximum concentration of 285 mg/L in shaken flasks. Statistical analysis showed that glycerol, NaNO3,MgSO4 and yeast extract had significant effects on production. The results were confirmed in a batchwise bioreactor, and semi-growth-associated production was detected. Reduction in the surface tension, which indicates the presence of biosurfactant, reached a value of 38 mN/m at the end of 35 hours. Use of the produced biosurfactant for washing crude oil-contaminated soil showed that 2 and 4 times the critical micellar concentration (CMC) were able to remove 97 and 99 percent of the oil, respectively, after 1 month of impregnation.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Archives / Biodegradation, Environmental / Rhodococcus / Petroleum / Chemical Industry / Contaminant Removal / Hydrocarbons Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio de Janeiro/BR

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Full text: Available Index: LILACS (Americas) Main subject: Archives / Biodegradation, Environmental / Rhodococcus / Petroleum / Chemical Industry / Contaminant Removal / Hydrocarbons Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2010 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio de Janeiro/BR