Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
Braz. j. microbiol
;
41(3): 796-804, Oct. 2010. graf, tab, ilus
Article
in English
| LILACS
| ID: lil-549427
ABSTRACT
A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinatequinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The á-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate quinone oxidoreductase activity was found to be high in this microorganism.
Full text:
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Index:
LILACS (Americas)
Main subject:
Oxidoreductases
/
Proteus Infections
/
Proteus mirabilis
/
Cell Membrane
/
Cytochromes b
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2010
Type:
Article
Affiliation country:
Pakistan
Institution/Affiliation country:
GC University/PK
/
Pakistan Council of Scientific and Industrial Research Labs Complex/PK
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