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Genetic and physical map of broad host range cosmid pRG930cm
Chibeu, Andrew; Lavigne, Rob; Mattheus, Wesley; Matthijs, Sandra; Cornelis, Pierre; Volckaert, Guido.
  • Chibeu, Andrew; Vrije Universiteit Brussel. Flanders Interuniversity Institute of Biotechnology. Department of Cellular and Molecular Interactions. Laboratory of Microbial Interactions. Brussels. BE
  • Lavigne, Rob; Katholieke Universiteit Leuven. Division of Gene Technology. Leuven. BE
  • Mattheus, Wesley; Katholieke Universiteit Leuven. Division of Gene Technology. Leuven. BE
  • Matthijs, Sandra; Vrije Universiteit Brussel. Flanders Interuniversity Institute of Biotechnology. Department of Cellular and Molecular Interactions. Laboratory of Microbial Interactions. Brussels. BE
  • Cornelis, Pierre; Vrije Universiteit Brussel. Flanders Interuniversity Institute of Biotechnology. Department of Cellular and Molecular Interactions. Laboratory of Microbial Interactions. Brussels. BE
  • Volckaert, Guido; Katholieke Universiteit Leuven. Division of Gene Technology. Leuven. BE
Electron. j. biotechnol ; 12(2): 10-11, Apr. 2009. ilus, tab
Article in English | LILACS | ID: lil-551371
ABSTRACT
We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No. FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries.
Subject(s)

Full text: Available Index: LILACS (Americas) Main subject: Pseudomonas / Cosmids / Agrobacterium tumefaciens / Azorhizobium caulinodans / Escherichia coli Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2009 Type: Article Affiliation country: Belgium Institution/Affiliation country: Katholieke Universiteit Leuven/BE / Vrije Universiteit Brussel/BE

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Full text: Available Index: LILACS (Americas) Main subject: Pseudomonas / Cosmids / Agrobacterium tumefaciens / Azorhizobium caulinodans / Escherichia coli Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2009 Type: Article Affiliation country: Belgium Institution/Affiliation country: Katholieke Universiteit Leuven/BE / Vrije Universiteit Brussel/BE