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Measuring b-Galactosidase activity at pH 6 with a differential pH sensor
Acevedo, Cristian; Stach, Matthias H; Amtmann, Anette; Young, Manuel E; Reyes, Juan G; Huebner, Holger; Buchholz, Rainer.
  • Acevedo, Cristian; Universidad Técnica Federico Santa María. Biotechnology Center. Valparaíso. CL
  • Stach, Matthias H; Friedrich-Alexander University of Erlangen-Nuremberg. Institute of Bioprocess Engineering. Erlangen. DE
  • Amtmann, Anette; Friedrich-Alexander University of Erlangen-Nuremberg. Institute of Bioprocess Engineering. Erlangen. DE
  • Young, Manuel E; Universidad Técnica Federico Santa María. Biotechnology Center. Valparaíso. CL
  • Reyes, Juan G; Pontificia Universidad Católica de Valparaíso. Chemistry Institute. Valparaíso. CL
  • Huebner, Holger; Friedrich-Alexander University of Erlangen-Nuremberg. Institute of Bioprocess Engineering. Erlangen. DE
  • Buchholz, Rainer; Friedrich-Alexander University of Erlangen-Nuremberg. Institute of Bioprocess Engineering. Erlangen. DE
Electron. j. biotechnol ; 12(2): 12-13, Apr. 2009. ilus, tab
Article in English | LILACS | ID: lil-551373
ABSTRACT
The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.
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Full text: Available Index: LILACS (Americas) Main subject: Beta-Galactosidase / Hydrogen-Ion Concentration Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2009 Type: Article / Project document Affiliation country: Chile / Germany Institution/Affiliation country: Friedrich-Alexander University of Erlangen-Nuremberg/DE / Pontificia Universidad Católica de Valparaíso/CL / Universidad Técnica Federico Santa María/CL

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Full text: Available Index: LILACS (Americas) Main subject: Beta-Galactosidase / Hydrogen-Ion Concentration Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2009 Type: Article / Project document Affiliation country: Chile / Germany Institution/Affiliation country: Friedrich-Alexander University of Erlangen-Nuremberg/DE / Pontificia Universidad Católica de Valparaíso/CL / Universidad Técnica Federico Santa María/CL