Boty-II, a novel LTR retrotransposon in Botrytis cinerea B05.10 revealed by genomic sequence
Electron. j. biotechnol
;
12(3): 2-3, July 2009. ilus, tab
Article
in English
| LILACS
| ID: lil-551880
ABSTRACT
Botrytis cinerea is a necrotrophic pathogen causing pre- and post-harvest diseases in at least 235 plant species. It manifests extraordinary genotype and phenotype variation. One of the causes of this variation is transposable elements. Two transposable elements have been discovered in this fungus, the retrotransposon (Boty), and the transposon (Flipper). In this work, two complete (Boty-II-76 and Boty-II-103) and two partial (Boty-II-95 and Boty-II-141) long terminal repeat (LTR) retrotransposons were identified by an in silico genomic sequence analysis. Boty-II-76 and Boty-II-103 contain 6439 bp nucleotides with a pair of LTRs at both ends, and an internal deduced pol gene encoding a polyprotein with reverse transcriptase and DDE integrase domains. They are flanked by 5 bp direct repeats (ACCAT, CTTTC). In Boty-II-141, two LTRs at both ends, and a partial internal pol gene encoding a protein with a DDE integrase domain were identified. In Boty-II-95, a right LTR and a partial internal pol gene encoding a protein with no conserved domains were identified. Boty-II uses a self-priming mechanism to initiate synthesis of reverse transcripts. The sequence of the presumed primer binding site for first-strand reverse transcription is 5-TTGTACCAT-3. The polypurine-rich sequence for plus-strand DNA synthesis is 5-GCCTTGAGCGGGGGGTAC-3. Fourteen Boty-II LTRs that contain 125-158 bp nucleotides and share 69.1 ~ 100 percent identities with the short inverted terminal repeats of 5 bp (TGTCA
TGACA) were discovered. Analysis of structural features and phylogeny revealed that Boty-II is a novel LTR retrotransposon. It could potentially be used as a novel molecular marker for the investigation of genetic variation in B. cinerea.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Retroelements
/
Botrytis
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2009
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Chinese Academy of Sciences/CN
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